Fifty-two people died and more than 700 were injured when a series of co-ordinated explosions hit three London Underground trains and a double-decker bus in 2005.
Marking the anniversary, Mr Khan offered his thoughts to the victims as well as his thanks to the city’s emergency services for their “heroic efforts” on that day.
He said: “Our capital stands together to reflect and remember the innocent victims, and our thoughts are with all those whose lives were changed forever.
“As we mark 16 years since the attacks on our city, I want again to pay tribute to the heroic efforts of our emergency services and transport workers, who ran towards danger to help people and save lives.
“The way our city responded that day and continues to stand united against terrorism shows the world that now and forever, those who seek to destroy our way of life in London will never win.
“London will always stand defiantly against the evils of hatred and terrorism. Our values of freedom, tolerance and mutual respect will always prevail over those who seek to divide us.”
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